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Synthesis of a porous hollow magnetic molecularly imprinted microsphere by O/W/O composite emulsion polymerization for specifically recognizing bovine serum albumin.
- Source :
-
Separation & Purification Technology . Jan2024, Vol. 329, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
-
Abstract
- • A porous hollow molecularly imprinted microsphere was synthesized by O/W/O composite emulsion polymerization. • The obtained microspheres were superparamagnetic and thermo-responsive. • The HMMIM adsorption capacity toward BSA was 89.9 mg/g and a good separation efficacy toward BSA was observed in bovine whole blood. Molecular imprinting technology is highly successful for low-molecular-weight molecules, but is still confronted with many challenges for proteins. This study introduces a strategy to synthesize hollow magnetic molecularly imprinted microspheres (HMMIMs) under Fe 3 O 4 magnetic nanoparticles matrix, by oil-in-water-in-oil composite emulsion polymerization with alkylphenol ethoxylates and Span-80 as emulsifiers. The synthesis conditions after optimization were 1.0 mmol thermo-sensitive functional monomer N-isopropylacrylamide, 1.0 mmol cross-linker N, N-methylene bisacrylamide, 2.0 wt% vinyl-modified silk fibroin, and 50 mg Fe 3 O 4 @COOH nanoparticles. The morphology structure and the forming process of the acquired HMMIMs were well characterized by POM, SEM, TEM and FT-IR. The HMMIMs were synthesized successfully with a porous structure and large specific surface area. The particle size of the obtained HMMIMs was at 10–20 µm. The obtained microspheres were superparamagnetic and thermo-responsive. The HMMIM adsorption capacity at 25 °C toward bovine serum albumin (BSA) was 89.9 mg/g and the BSA separation factor in BSA/BHb mixtures was 2.12. A good separation efficacy toward BSA was also observed in bovine whole blood. The prepared HMMIM possessed heterogeneous active sites, the BSA adsorption process on HMMIM was chemisorption controlled. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13835866
- Volume :
- 329
- Database :
- Academic Search Index
- Journal :
- Separation & Purification Technology
- Publication Type :
- Academic Journal
- Accession number :
- 173691929
- Full Text :
- https://doi.org/10.1016/j.seppur.2023.125197