circRNAs deregulation in exosomes derived from BEAS-2B cells is associated with vascular stiffness induced by PM2.5.

• Differential exosomal circRNAs and mRNAs expression profiles after PM 2.5 treatment. • ExoPM2.5 caused endothelial dysfunction by permeability and decreased relaxation. • hsa_circ_0012627, hsa_circ_0053261 and hsa_circ_0052810 may predict vascular injury. • Vascular stiffness caused by PM 2.5 may be partly due to exosomal circRNAs from lung. As an environmental pollutant, ambient fine particulate matter (PM 2.5) was linked to cardiovascular diseases. The molecular mechanisms underlying PM 2.5 -induced extrapulmonary disease has not been elucidated clearly. In this study the ambient PM 2.5 exposure mice model we established was to explore adverse effects of vessel and potential mechanisms. Long-term PM 2.5 exposure caused reduced lung function and vascular stiffness in mice. And chronic PM 2.5 induced migration and epithelial-mesenchymal transition (EMT) phenotype in BEAS-2B cells. After PM 2.5 treatment, the circRNAs and mRNAs levels of exosomes released by BEAS-2B cells were detected by competing endogenous RNA (ceRNA) array, which contained 1664 differentially expressed circRNAs (DE-circRNAs) and 308 differentially expressed mRNAs (DE-mRNAs). By bioinformatics analysis on host genes of DE-circRNAs, vascular diseases and some pathways related to vascular diseases including focal adhesion, tight junction and adherens junction were enriched. Then, ceRNA network was constructed, and DE-mRNAs in ceRNA network were conducted functional enrichment analysis by Ingenuity Pathway Analysis, which indicated that hsa_circ_0012627, hsa_circ_0053261 and hsa_circ_0052810 were related to vascular endothelial dysfunction. Furthermore, it was verified experimentally that ExoPM2.5 could induce endothelial dysfunction by increased endothelial permeability and decreased relaxation in vitro. In present study, we investigated in-depth knowledge into the molecule events related to PM 2.5 toxicity and pathogenesis of vascular diseases. [Display omitted] [ABSTRACT FROM AUTHOR]