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Vitamin D3 mediated peptides-calcium chelate self-assembly: Fabrication, stability and improvement on cellular calcium transport.
- Source :
-
Food Chemistry . Mar2024:Part 1, Vol. 437, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
-
Abstract
- • A peptides-calcium-VD 3 ternary delivery system (CSPH-Ca-VD 3) was prepared self-assembly. • Hydrophobic interaction was determined to be the major driving force. • CSPH-Ca-VD 3 synchronously prevented acid degradation of VD 3 and improved calcium solubility. • CSPH-Ca-VD 3 promoted calcium transcellular transport through TRPV6 channel. A self-assembled peptides-calcium-Vitamin D 3 ternary delivery system (CSPH-Ca-VD 3) was prepared to investigate the promotion of cellular calcium transport. The constructed CSPH-Ca-VD 3 nanocomplex exhibited a spherical structure with a size of 135.2 ± 10.2 nm. Based on the thermodynamic calculation of fluorescent spectra, hydrophobic interaction was shown as the major driving force for this nanocomplex structure. CSPH-Ca-VD 3 nanocomplex possessed excellent stability during simulated gastrointestinal digestion, contributing to the prevention of acid degradation of VD 3 and the enhancement of calcium solubility. Furthermore, the calcium transport efficiency in the form of CSPH-Ca-VD 3 (4 mg/mL) across a Caco-2 cells monolayer was significantly increased 2.3-fold compared to that of free Ca2+, mainly attributed to the upregulation in the presence of CSPH-Ca-VD 3 of TRPV6, calbindin D9k and PMCA1b expression in Caco-2 cells. The present study provided a basis for developing a novel delivery system of peptides-calcium chelate with the dual effects of VD 3 protection and calcium uptake promotion. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03088146
- Volume :
- 437
- Database :
- Academic Search Index
- Journal :
- Food Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 173706184
- Full Text :
- https://doi.org/10.1016/j.foodchem.2023.137779