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Convergent synthesis of phosphorodiamidate morpholino oligonucleotides (PMOs) by the H-phosphonate approach.
- Source :
-
Scientific Reports . 11/18/2023, Vol. 13 Issue 1, p1-10. 10p. - Publication Year :
- 2023
-
Abstract
- Phosphorodiamidate morpholino oligonucleotides (PMOs) are a promising type of antisense oligonucleotides, but their challenging synthesis makes them difficult to access. This research presents an efficient synthetic approach for PMOs using the H-phosphonate approach. The use of phosphonium-type condensing reagents significantly reduced coupling times compared with the current synthetic approach. Furthermore, phosphonium-type condensing reagents facilitated the fragment condensation of PMO, synthesizing up to 8-mer containing all four nucleobases with remarkable coupling efficacy. This is the first report on the convergent synthesis of PMOs. This approach would facilitate the large-scale synthesis of PMOs and accelerate their popularity and accessibility as a next-generation therapy. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BASE pairs
*PHOSPHONATES
*CONDENSATION
*POPULARITY
*OLIGONUCLEOTIDES
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 13
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Scientific Reports
- Publication Type :
- Academic Journal
- Accession number :
- 173737473
- Full Text :
- https://doi.org/10.1038/s41598-023-38698-2