Integrated FET sensing microsystem for specific detection of pancreatic cancer exosomal miRNA10b.

Tumor-derived exosome (TD-Ex) serves as a crucial early diagnostic biomarker of pancreatic cancer (PC). However, accurate identification of TD-Ex from PC is still a challenging work. In this paper, a detection microsystem that integrates magnetic separation and FET biosensor is developed, which is capable of selectively separating TD-Ex of PC from the plasma and detecting exosomal miRNA10b in a sensitive and specific manner. The magnetic beads were functionalized with dual antibody (GPC-1 antibody and EpCAM antibody), enabling selective recognition and capture of PC-derived exosomes. On the other hand, a peptide nucleic acid (PNA)- functionalized reduced graphene oxide field-effect transistor (RGO FET) biosensor was subsequently utilized to detect the exosomal miRNA10b, which is highly expressed in PC- derived exosomes. This system could achieve a low detection limit down to 78 fM, and selectively identify miRNA10b from single-base mismatched miRNA. In addition, 40 clinical plasma samples were tested with this microsystem, and the results indicate that it could effectively distinguish PC patients from healthy individuals. The assay combines specific capture and enrichment of PC-derived exosomes with sensitive and selective detection of exosomal miRNA, showing its potential to be used as an effective scheme for PC early diagnosis. An integrated microsystem is depicted, designed for the specific isolation and enrichment of pancreatic cancer-derived exosomes via dual-antibody functionalized magnetic beads. Simultaneously, the utilization of a Peptide Nucleic Acid (PNA)-functionalized Field-Effect Transistor (FET) biosensor for the identification of exosomal miRNA10b is represented. This highlights the microsystem's enhanced sensitivity and selectivity, along with its capability for label-free, rapid detection, facilitating clear differentiation between pancreatic cancer patients and healthy individuals. [Display omitted] • A novel magnetic beads-based assay is constructed to isolate specific exosome. • Exosomal miRNA10b using a FET biosensor is sensitively detected. • A microsystem integrating exosome-selective isolation and detection is established. • The microsystem accurately distinguishes PC patients from healthy individuals. • An integrated microsystem is developed for early diagnosis of tumors. [ABSTRACT FROM AUTHOR]