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A new simple fluorescent probe for imaging endogenous hydrogen peroxide in RAW 264.7 cells.

Authors :
Wei, Huiqin
Chen, Huijia
Liu, Shun
Tan, Meixia
Zhang, Zhiqiang
Xu, Xiuzhi
Ke, Fang
Source :
Tetrahedron Letters: International Organ for the Rapid Publication of Preliminary Communications in Organic Chemistry. Nov2023, Vol. 132, pN.PAG-N.PAG. 1p.
Publication Year :
2023

Abstract

[Display omitted] One of the most significant species that are present in large quantities in biological cells is reactive oxygen species (ROS). They are essential for cell motility, cell differentiation, and cellular immunity. In this paper, we introduce that (E)-1-methyl-4-(4-(propionyloxy)styryl)pyridin-1-ium (MPSPI) is an "on" type fluorescent probe regulated by the ICT mechanism. After the addition of hydrogen peroxide, the fluorophore (E)-4-(4-hydroxystyryl)-1-methylpyridin-1-ium (HMI-OH) is released. The simple probe MPSPI not only exhibited good selectivity and low cytotoxicity, but also demonstrated a detection limit as low as 35 nM and reaction times as quick as 20 s. Moreover, it has been successfully applied to the imaging of hydrogen peroxide in RAW 264.7 cells. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00404039
Volume :
132
Database :
Academic Search Index
Journal :
Tetrahedron Letters: International Organ for the Rapid Publication of Preliminary Communications in Organic Chemistry
Publication Type :
Academic Journal
Accession number :
173783815
Full Text :
https://doi.org/10.1016/j.tetlet.2023.154818