Characterization of a Novel Antibody Drug Conjugate Mimic by Size Exclusion and Hydrophobic Interaction Chromatography.

The article delves into the study of antibody-drug conjugates (ADCs), emphasizing the importance of understanding the drug-to-antibody ratio (DAR) for both drug efficacy and clearance during the development of biotherapeutic drug candidates. The research employs size exclusion chromatography/mass spectrometry (SEC/MS) and hydrophobic interaction chromatography (HIC) to characterize the DAR profile of an ADC mimic.