马尾藻多糖拮抗LPS 诱导的巨噬细胞极化及铁死亡.

Objective: To study the protective effect of sargassum polysaccharides （SP） on macrophage polarization and iron death induced by lipopolysaccharide （LPS） in order to determine whether SP has a regulatory effect on immune function. Methods: Isolating, purificating and identificating SP, a mouse macrophage RAW264.7 cell line inflammation model induced by LPS was established. The CCK-8 method was used to detect the effect of SP on macrophage proliferation. The levels of CD80, CD163, IL-6, IL-10, Arginine-1 （Arg-1）, inducible nitric oxide synthase （iNOS）, IL-6 and TNF-α in macrophages were measured by real-time fluorescence quantitative PCR （RT-qPCR） and Western blot. In order to understand the iron death of macrophages, ROS content was detected with ROS assay kit, and glutathione peroxid 4（ GPX4） expression was detected by Western blot. Results: SP were isolated successfully and identified not as furanose. High concentration of SP promote the proliferation of macrophages and had a significant protective effect on the inhibition of cell proliferation induced by LPS. SP could polarize RAW264.7 cells to M2 and antagonize M1 polarization induced by LPS. LPS could significantly increase ROS in macrophages, while SP could significantly decrease the level of ROS. The expression of GPX4 protein in macrophages induced by LPS was significantly decreased, and the expression was significantly up-regulated by high concentration of SP （P<0.05）. Conclusion: SP induces macrophages M2 polarization and antagonizes LPS induced M1 polarization and ferroptosis of macrophages. [ABSTRACT FROM AUTHOR]