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DNA metabarcoding focusing on the plankton community: an effective approach to reconstruct the paleo-environment.
DNA metabarcoding focusing on the plankton community: an effective approach to reconstruct the paleo-environment.
- Source :
-
Scientific Reports . 12/7/2023, Vol. 13 Issue 1, p1-8. 8p. - Publication Year :
- 2023
-
Abstract
- DNA metabarcoding (DNA-MB) targeting the whole plankton community is a promising approach in studies of sediment samples from water bodies, but its effectiveness in ancient material is not well demonstrated. We applied DNA-MB of plankton in a sediment core to reconstruct the paleo-environment of Lake Shinji, Japan, through a marine lagoon/freshwater lake transition during the past 2300 years. We interpreted core-sample plankton taxonomy and habitat by reference to the modern plankton community in water samples. OTUs (operational taxonomic units) belonging to Dictyochophyceae were 81.05% of the total reads in sediments. However, Ciliophora, Copepoda and Labyrinthulea formed the majority of plankton taxa in the water samples, suggesting that they are under-represented in sediment. A drastic change in plankton composition correlated with a large decrease in sediment sulfur concentration, implying the change of aquatic environment from marine lagoon to freshwater lake. This event took place ca. 1200 CE in Lake Shinji. A 250 year-long transitional period followed, during which the total DNA sequence reads were very low. This suggests that salinity fluctuations created a hostile environment for both marine and freshwater plankton species. Our results show that DNA-MB of the whole plankton community is effective in reconstructing paleo-environments. [ABSTRACT FROM AUTHOR]
- Subjects :
- *GENETIC barcoding
*LAKES
*PLANKTON
*MARINE plankton
*BODIES of water
*ECOLOGY
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 13
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Scientific Reports
- Publication Type :
- Academic Journal
- Accession number :
- 174095572
- Full Text :
- https://doi.org/10.1038/s41598-023-48367-z