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A Time-resolved Investigation of Ribosomal Subunit Association
- Source :
-
Journal of Molecular Biology . Mar2005, Vol. 346 Issue 5, p1243-1258. 16p. - Publication Year :
- 2005
-
Abstract
- The notion that the ribosome is dynamic has been supported by various biochemical techniques, as well as by differences observed in high-resolution structures of ribosomal complexes frozen in various functional states. Yet, the mechanisms and extent of rRNA dynamics are still largely unknown. We have used a novel, fast chemical-modification technique to provide time-resolved details of 16S rRNA structural changes that occur as bridges are formed between the ribosomal subunits as they associate. Association of different 16S rRNA regions was found to be a sequential, multi-step process involving conformational rearrangements within the 30S subunit. Our results suggest that key regions of 16S rRNA, necessary for decoding and tRNA A-site binding, are structurally altered in a time-dependent manner by association with the 50S ribosomal subunits. [Copyright &y& Elsevier]
- Subjects :
- *RIBOSOMES
*TRANSFER RNA
*ELECTRON microscopy
*RNA
*ORGANELLES
Subjects
Details
- Language :
- English
- ISSN :
- 00222836
- Volume :
- 346
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Journal of Molecular Biology
- Publication Type :
- Academic Journal
- Accession number :
- 17419047
- Full Text :
- https://doi.org/10.1016/j.jmb.2004.12.054