Production of bacterial cellulose on hydrolysates of vegetable raw materials and practical aspects of its use.

The possibility of using enzymatic hydrolysate of Chinese miscanthus cellulose as a substrate for bacterial cellulose biosynthesis has been studied. It was shown that when an enzymatic hydrolysate was introduced into the nutrient medium in the amount of 20 g/l, the productivity of bacterial cellulose by the Komagataebacter xylinus B-12431 strain was 4.6 ± 0.23 g/l. The influence of temperature and duration of cultivation on the productivity of bacterial cellulose has been studied. The maximum yield of bacterial cellulose was observed after 14 days of cultivation at a temperature of 28 ± 0.5 ° C. The analysis of the physico-chemical properties of bacterial cellulose was carried out. It was found that bacterial cellulose does not contain impurities of lignin and hemicelluloses, the mass fraction of acid-insoluble lignin is less than 0.1%, the degree of crystallinity of bacterial cellulose reaches 80%. The resulting bacterial cellulose is characterized by a high degree of polymerization and a high content of alpha-cellulose. Based on the studied physico-chemical characteristics, practical recommendations for the use of bacterial cellulose in chemical, food, biotechnological, biomedical industries have been determined. Bacterial cellulose can be in demand as a stabilizer, emulsifier and thickener in food production, for the production of packaging materials, in the production of biofilters and for the immobilization of enzyme preparations and bacterial cells, can be widely used in medicine as a new material for the manufacture of bone precursor, artificial cartilage, dressings, as well as the manufacture of matrix-a carrier for pharmaceutical substances. [ABSTRACT FROM AUTHOR]