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Explore the anti-inflammation mechanism of safflower total flavonoids in the treatment of endometritis based on celluar transcriptomics.
- Source :
-
Journal of Pharmacy & Pharmacology . Nov2023, Vol. 75 Issue 11, p1478-1491. 14p. - Publication Year :
- 2023
-
Abstract
- Objectives: Safflower is a traditional Chinese medicine for the treatment of gynecological diseases and its flavonoids have potential anti-inflammatory effects. The purpose is to explore the possible effects of safflower total flavonoids (STF) on lipopolysaccharide (LPS)-induced inflammatory damage of Ishikawa cells. Method: In this study, LPS-induced endometrial carcinoma Ishikawa cells were used to establish an inflammatory injury model. The effective concentration of STF was screened by CCK-8 and enzyme-linked immunosorbent assay. The apoptosis of damaged Ishikawa cells was detected by flow cytometry. The contents of caspase11 and caspase 3 in Ishikawa cells were observed by fluorescence imaging. Western blot and RT-qPCR were used to detect the expression of related proteins and mRNA in damaged Ishikawa cells, and the possible mechanism of safflower flavonoids against LPS-induced endometrial carcinoma Ishikawa cells was analyzed by cell transcriptomics. Key findings: The STF-reduced tumor necrosis factor α, interleukin-1β, and interleukin-6 expression level; the expression level of the proteins ASK1, Caspase3, and Caspase11 was also significantly decreased, and the proteins ERα, p-PI3K, and p-AKT were significantly increased. The transcriptome results showed that the PI3K-Akt signal pathway may be the main signal pathway for the STF. Conclusion: The STF could regulate the PI3K/AKT signal pathway to treat the inflammatory injury of Ishikawa cells. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00223573
- Volume :
- 75
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Journal of Pharmacy & Pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 174274717
- Full Text :
- https://doi.org/10.1093/jpp/rgad082