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Sporotrichum thermophile type C feruloyl esterase (StFaeC): purification, characterization, and its use for phenolic acid (sugar) ester synthesis

Authors :
Topakas, E.
Vafiadi, C.
Stamatis, H.
Christakopoulos, P.
Source :
Enzyme & Microbial Technology. Apr2005, Vol. 36 Issue 5/6, p729-736. 8p.
Publication Year :
2005

Abstract

Abstract: A feruloyl esterase (StFaeC) produced by Sporotrichum thermophile was purified to homogeneity. The native StFaeC was homodimer with a subunit of M r 23,000 and pI 3.1. The enzyme activity was optimal at pH 6.0 and 55°C. The esterase displayed remarkable stability at pH 10.0 and retained 50% of its activity after 133 and 55min at 55 and 60°C, respectively. Determination of k cat/K m revealed that the enzyme had a broad spectrum of activity against the (hydroxyl) cinnamate esters indicating a type C feruloyl esterase. The enzyme was active on substrates containing ferulic acid ester linked to the C-5 and C-2 linkages of arabinofuranose and hydrolysed 4-nitrophenyl-5-O-trans-feruloyl-α-l-arabinofuranoside three times more efficiently than 4-nitrophenyl-2-O-trans-feruloyl-α-l-arabinofuranoside. Ferulic acid was efficiently released from wheat bran when the esterase was incubated together with xylanase from S. thermophile (a maximum of 41% total ferulic acid released after 1h incubation). StFaeC by itself could release FA but at a level almost 10-fold lower than that obtained in the presence of xylanase. The potential of StFaeC for the synthesis of various phenolic acid esters was examined using as a reaction system a ternary water–organic mixture consisting of n-hexane, 1-butanol and water. Also StFaeC catalyzed the transfer of the feruloyl group to l-arabinose in a similar system using t-butanol, with about a 40% conversion of l-arabinose to feruloylated derivative was achieved. This work is the first example of enzymatic feruloylation of a carbohydrate. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
01410229
Volume :
36
Issue :
5/6
Database :
Academic Search Index
Journal :
Enzyme & Microbial Technology
Publication Type :
Academic Journal
Accession number :
17436706
Full Text :
https://doi.org/10.1016/j.enzmictec.2004.12.020