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gRNA Design: How Its Evolution Impacted on CRISPR/Cas9 Systems Refinement.
- Source :
-
Biomolecules (2218-273X) . Dec2023, Vol. 13 Issue 12, p1698. 21p. - Publication Year :
- 2023
-
Abstract
- Over the past decade, genetic engineering has witnessed a revolution with the emergence of a relatively new genetic editing tool based on RNA-guided nucleases: the CRISPR/Cas9 system. Since the first report in 1987 and characterization in 2007 as a bacterial defense mechanism, this system has garnered immense interest and research attention. CRISPR systems provide immunity to bacteria against invading genetic material; however, with specific modifications in sequence and structure, it becomes a precise editing system capable of modifying the genomes of a wide range of organisms. The refinement of these modifications encompasses diverse approaches, including the development of more accurate nucleases, understanding of the cellular context and epigenetic conditions, and the re-designing guide RNAs (gRNAs). Considering the critical importance of the correct performance of CRISPR/Cas9 systems, our scope will emphasize the latter approach. Hence, we present an overview of the past and the most recent guide RNA web-based design tools, highlighting the evolution of their computational architecture and gRNA characteristics over the years. Our study explains computational approaches that use machine learning techniques, neural networks, and gRNA/target interactions data to enable predictions and classifications. This review could open the door to a dynamic community that uses up-to-date algorithms to optimize and create promising gRNAs, suitable for modern CRISPR/Cas9 engineering. [ABSTRACT FROM AUTHOR]
- Subjects :
- *GENOME editing
*CRISPRS
*GENETIC engineering
*MACHINE learning
*NUCLEASES
*GENOMES
Subjects
Details
- Language :
- English
- ISSN :
- 2218273X
- Volume :
- 13
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- Biomolecules (2218-273X)
- Publication Type :
- Academic Journal
- Accession number :
- 174401701
- Full Text :
- https://doi.org/10.3390/biom13121698