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A novel strategy for specific sensing and inactivation of Escherichia coli: Constructing a targeted sandwich-type biosensor with multiple SERS hotspots to enhance SERS detection sensitivity and near-infrared light-triggered photothermal sterilization performance
- Source :
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Talanta . Mar2024, Vol. 269, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
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Abstract
- Human health is greatly threatened by bacterial infection, which raises the risk of serious illness and death in humans. For early screening and accurate treatment of bacterial infection, there is a strong desire to undertake ultrasensitive detection and effective killing of pathogenic bacteria. Herein, a novel surface-enhanced Raman scattering (SERS) biosensor based on sandwich structure consisting of capture probes/bacteria/SERS tags was established for specific identification, capture and photothermal killing of Escherichia coli (E. coli). Finite-difference time-domain (FDTD) technique was used to simulate the electromagnetic field distribution of capture probes, SERS tags and sandwich-type SERS substrate, and a possible SERS enhancement mechanism based on sandwich structure was presented and discussed. Sandwich-type SERS biosensor successfully achieved distinctive identification and magnetic beneficiation of E. coli. In addition, a single SERS substrate, including capture probes and SERS tags, could also achieve outstanding photothermal effects as a consequence of localized surface plasmon resonance (LSPR) effect. Intriguingly, sandwich-type SERS biosensor demonstrated a higher photothermal conversion efficiency (50.03 %) than the single substrate, which might be attributed to the formation of target bacterial clusters. The superior biocompatibility and the low toxicity of the sandwich-type biosensor were confirmed. Our approach offers a fresh method for constructing sandwich-type biosensor with multiple SERS hotspots based on extremely effective hybrid plasmonic nanoparticles, and has a wide range of potential applications in the recognition and treatment of bacteria. [Display omitted] • SERS platform for simultaneous detection and inactivation of E. coli was developed. • A dual SERS enhancement strategy based on sandwich-type structure was applied. • The limit of detection for E. coli was as low as 10 cfu/mL. • Photothermal conversion efficiency of the SERS platform was as high as 50.03 %. • SERS biosensor had an excellent photothermal performance for killing of E. coli. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00399140
- Volume :
- 269
- Database :
- Academic Search Index
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 174469821
- Full Text :
- https://doi.org/10.1016/j.talanta.2023.125466