Distribution of cellulose degrading bacteria from Imperata cylindrical grasslands in Indonesia.

During our study on isolation and screening cellulolytic degrading bacteria from three different Imperata cylindrica grasslands in Indonesia (Bogor, West Java; Katingan, Central Kalimantan; and Malaka, East Nusa Tenggara), we obtained 18 isolates. This study aims to investigate the biodiversity of cellulolytic degrading bacteria from three locations of I. cylindryca grassland in Indonesia by identifying those 18 bacteria based on 16S rRNA gene sequencing. As a result, the phylogenetic analysis based on 16S rRNA gene sequence indicated that all isolates belonged to ten genera that consisted of Bacillus (5), Burkholderia (2), Enterobacter (3), Lysobacter (1), Microbacterium (1), Mitsuaria (1), Novosphingobium (1), Pedobacter (1), Paenibacillus (2), and Paraburkholderia (1). The phylogenetic tree revealed that seven isolates isolated from (RM) East Nusa Tenggara were identified as Mitsuaria chitinivorans RM165, Lysobacter enzymogenes RM158, Enterobacter sp. RM20, Novosphingobium sp. RM155, Pedobacter sp. RM40, Microbacterium zeae RM157 and Bacillus sp. RM164, six isolates isolated from Katingan (East Kalimantan) were identified as Burkholderia sp. KAT225, Enterobacter sp. KAT82, Enterobacter sp. KAT88, Bacillus sp. KAT80, Paenibacillus kribbensis KAT83, and Paenibacillus sp. KAT15, and five isolates isolated from Cibinong (West Java) were identified as Burkholderia sp. CSC185, Paraburkholderia caribensis CSC177, Bacillus sp. CSC179, Bacillus sp. CSC187 and Bacillus sp. CSC183. This finding revealed that various genera from the Phylum of Pseudomonadota, Bacillota, Bacteroidota, and Actinomycetota have the capability to produce cellulase. Further study on the quantitative analysis of the cellulase enzyme measurement is needed. [ABSTRACT FROM AUTHOR]