Decagram-Scale Synthesis of N -{2-[4-(β- d -Glucopyranosyloxy)-2-methylphenyl]-1,1-dimethyl-2-oxoethyl}-3-methylthiophene-2-carboxamide (GPTC), a Metabolite of the Fungicide Isofetamid.

This article discusses the synthesis of N-{2-[4-(β-d-Glucopyranosyloxy)-2-methylphenyl]-1,1-dimethyl-2-oxoethyl}-3-methylthiophene-2-carboxamide (GPTC), a metabolite of the fungicide isofetamid. The authors describe the importance of certified reference materials (CRMs) for accurate analysis of toxic chemicals in food. They report a decagram-scale synthesis of GPTC to obtain a standard CRM for residue analysis in food. The synthesis involves several steps, including protection, amination, coupling, and glycosylation, resulting in the production of 25 grams of GPTC with a purity of 99.6%. The authors conclude that this synthetic approach can be used to establish a standard CRM for analyzing residues in food. [Extracted from the article]