PHYTOCHEMICAL SCREENING, ANTIOXIDANT ACTIVITY, ANTI-INFLAMMATORY, TOXICITY USING BRINE SHRIMP LETHALITY TEST OF VARIATION EXTRACTS OF Senna alata L. LEAF EXTRACT.

This study determined phytochemical screenings, antioxidant activity, anti-inflammatory, and toxicity of Senna alata L. leaf extract by using several method extractions that as maceration (A), reflux (B), and Ultrasonic Assist Extraction (UAE) (C). Phytochemical screening was obtained that leaves extract of Senna alata L. variations A, B, and C contained alkaloids, flavonoids, phenolics, terpenoids, and saponins. FRAP test was used to determine antioxidant activity and IC 50. It was obtained IC50 < 50 ppm and classed as a potent antioxidant. The anti-inflammatory activity of each Senna alata L leaf extract was obtained with IC50 < 50 ppm. The Bhrine Shrimp Lethality Test (BSLT) was utilized to test toxicity by calculating the LC50 value. It was obtained that the LC50 value of Senna alata L. leaf extracts <1000 ppm showed that Senna alata L. leaf extracts are toxic to Artemia salina Leach larvae so it is estimated to have anticancer potential. The highest toxicity (LC50) was Senna alata L. leaf extracts by variation C 235.79 mg/L. Variation C is Senna alata L. leaf extract using the Ultrasonic Assist Extraction (UAE) method. [ABSTRACT FROM AUTHOR]