Back to Search
Start Over
ULK1 confers neuroprotection by regulating microglial/macrophages activation after ischemic stroke.
- Source :
-
International Immunopharmacology . Jan2024, Vol. 127, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
-
Abstract
- • ULK1 was up-regulated in infarct region and mainly expressed in microglia following ischemic injury. • Pharmacological ULK1 activation ameliorated behavioral disorders, and improved neuronal recovery after ischemic injury. • Pharmacological ULK1 activation promoted microglia switching to anti-inflammatory phenotype after ischemic injury. Microglial activation and autophagy play a critical role in the progression of ischemic stroke and contribute to the regulation of neuroinflammation. Unc-51-like kinase 1 (ULK1) is the primary autophagy kinase involved in autophagosome formation. However, the impact of ULK1 on neuroprotection and microglial activation after ischemic stroke remains unclear. In this study, we established a photothrombotic stroke model, and administered SBI-0206965 (SBI), an ULK1 inhibitor, and LYN-1604 hydrochloride (LYN), an ULK1 agonist, to modulate ULK1 activity in vivo. We assessed sensorimotor deficits, neuronal apoptosis, and microglial/macrophage activation to evaluate the neurofunctional outcome. Immunofluorescence results revealed ULK1 was primarily localized in the microglia of the infarct area following ischemia. Upregulating ULK1 through LYN treatment significantly reduced infarct volume, improved motor function, promoted the increase of anti-inflammatory microglia. In conclusion, ULK1 facilitated neuronal repair and promoted the formation of anti-inflammatory microglia pathway after ischemic injury. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MICROGLIA
*ISCHEMIC stroke
*MACROPHAGE activation
*COGNITIVE therapy
*AUTOPHAGY
Subjects
Details
- Language :
- English
- ISSN :
- 15675769
- Volume :
- 127
- Database :
- Academic Search Index
- Journal :
- International Immunopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 174760680
- Full Text :
- https://doi.org/10.1016/j.intimp.2023.111379