Screening of factors influencing riboflavin producing recombinant Ashbya Gossypii using Plackett-Burman fractional factorial design experiment.

Riboflavin is an intrinsic component of significant nutritional and growth factors of living organisms. It was fermentatively synthesized from different microorganisms such as Ashbya gossypii, Eremothecium ashbyii, Candida famata, and Bacillus subtilis. This water-soluble vitamin is a part of commercial interest since, the earliest decade when human health was in threat. Despite its multiple utilities in all market sectors, the global riboflavin market is seeming to increase its demand, annually. To that, findings affecting to various environmental and nutritional factors contributing to riboflavin yield were improvised with a cost-effective statistical experimental design. Previously, the individual effect of components on riboflavin production was evaluated via One-Factor-at-a-Time (OFAT) method. Using recombinant Ashbya gossypii strain, the combined effects of six variables (sunflower oil, corn steep liquor, yeast extract, glycine, potassium phosphate, and Tween 60) were assessed in Plackett-Burman Design. A total of five statistically significant (P<0.05) factors - sunflower oil, Tween 60, corn steep liquor, glycine, and potassium phosphate (KH2PO4) were identified. The statistical analysis proposed that the linear mathematical model used for prediction is significant with the coefficient of determination R2(0.9025). Comparatively to the original medium composition, 49% of higher metabolite was achieved based on the model of the optimized medium. [ABSTRACT FROM AUTHOR]