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Cost-effective production of ergothioneine using Rhodotorula mucilaginosa DL-X01 from molasses and fish bone meal enzymatic hydrolysate.
- Source :
-
Bioresource Technology . Feb2024, Vol. 393, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
-
Abstract
- [Display omitted] • Untreated molasses could be utilized by R. mucilaginosa DL-X01 as carbon source. • Fish bone meal enzymatic hydrolysate was used to replace yeast extract and peptone. • The amount of molasses and fish bone meal added were optimized. • Feedstock cost of producing EGT is reduced by 331 % compared to using YPD broth. Ergothioneine (EGT) is a high-value natural antioxidant that cannot be synthesized by the human body. This study showed that Rhodotorula mucilaginosa DL-X01 can use untreated molasses and fish bone meal enzymatic hydrolysate as the substrates to synthesize EGT. By optimizing the growth conditions, the EGT yield reached 29.39 mg/L when molasses and fish bone meal (FBM) were added at 60 g/L and 400 g/L respectively. Finally, the EGT yield was increased to 216.25 mg/L by fed-batch fermentation in a 5 L bioreactor. Compared with the fermentation by yeast extract peptone dextrose medium, the feedstock cost of EGT production was reduced by 330.91 % by using molasses and FBM as substrates. These results showed that R. mucilaginosa DL-X01 can produce high-value EGT using two cheap processing by-products, molasses and FBM, which is of great significance for environmental protection and sustainable development. [ABSTRACT FROM AUTHOR]
- Subjects :
- *FISH meal
*MOLASSES
*RHODOTORULA
*YEAST extract
*INDUSTRIAL costs
Subjects
Details
- Language :
- English
- ISSN :
- 09608524
- Volume :
- 393
- Database :
- Academic Search Index
- Journal :
- Bioresource Technology
- Publication Type :
- Academic Journal
- Accession number :
- 174792008
- Full Text :
- https://doi.org/10.1016/j.biortech.2023.130101