The potential mechanism of response to light intensity in energy metabolism mediated by miRNA in Isatis indigotica.

• Strong light promoted the energy metabolism of Isatis indigotica Fortune. • Light intensity affected the expression of 89 miRNAs and 16,485 mRNAs. • 12 miRNA-mRNA pairs play important roles in mitochondrial biological processes. • Light intensity affects 4 mitochondria-associated miRNA-mRNA pairs. Light is the main source of energy for plant growth. Studies have shown that I. indigotica is a light-demanding plant and its yield and various active components are positively correlated with light intensity, but no studies of light intensity affecting energy metabolism in I. indigotica have been reported. Mitochondria are the main site of energy metabolism, and miRNAs are important factors in regulating gene expression, this experiment attempts to study the effects of different light intensities on energy metabolism from the perspective of mitochondria and miRNAs. The results show that the biomass、mitochondrial structural integrity and energy metabolism in I. indigotica were found to be positively correlated with light intensity. Small RNA and transcriptome sequencing identified 241 miRNAs and 36,372 mRNAs, and degradomic technology identified 72 miRNAs targeting 106 mRNAs, among which 12 pairs of miRNA-mRNAs were annotated on mitochondria. Combined with RT-qPCR validation, it was concluded that miR167a-5p positively regulates LETM1 and affects mitochondrial structure, miR400-5p and mIR169m-p3_1ss15CT negatively regulate GRXS15 and CMC4 , respectively, affecting SDH and CCO activities, and miR395a- APS4 may affect the utilization of ATP and sulfate assimilation. In summary, the results of this study complement and enrich knowledge of light effects on mitochondria from the perspective of miRNA, while providing guidance for the cultivation of I. indigotica. [ABSTRACT FROM AUTHOR]