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High glucose-induced injury in human umbilical vein endothelial cells is alleviated by vitamin D supplementation through downregulation of TIPE1.
- Source :
-
Diabetology & Metabolic Syndrome . 1/13/2024, Vol. 16 Issue 1, p1-12. 12p. - Publication Year :
- 2024
-
Abstract
- Background: Diabetes mellitus (DM) and its associated vascular complications have become a worldwide health concern. The effects and mechanism of vitamin D supplementation on endothelial function under high glucose condition remain elusive. Methods: Human umbilical vein endothelial cells (HUVECs) were treated with 35 mM glucose, then 100 nM vitamin D were added. Transwell migration assay, CCK-8, immunofluorescence, flow cytometry, autophagy flux and transmission electric microscope were performed. Results: Vitamin D reduced apoptosis, promoted migration and enhanced viability of HUVECs, decreased TIPE1 (Tumor necrosis factor-α-induced protein 8-like 1) under high glucose conditions. Overexpression of TIPE1 reverses the effects of vitamin D by increasing ROS production, inflammation, cell apoptosis, and suppressing autophagy, cell migration and viability. And vitamin D negatively correlated with TIPE1 mRNA level in DM patients. Conclusions: Vitamin D reverses the harmful effects of high glucose on HUVECs by reducing TIPE1 expression. And vitamin D supplementation could help to alleviate high glucose-induced injury in type 2 diabetes mellitus patients with microvascular complications. [ABSTRACT FROM AUTHOR]
- Subjects :
- *VITAMIN D
*DIETARY supplements
*UMBILICAL veins
*ENDOTHELIAL cells
*TYPE 2 diabetes
Subjects
Details
- Language :
- English
- ISSN :
- 17585996
- Volume :
- 16
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Diabetology & Metabolic Syndrome
- Publication Type :
- Academic Journal
- Accession number :
- 174798949
- Full Text :
- https://doi.org/10.1186/s13098-024-01264-5