万寿菊叶黄素对蛋鸡蛋品质、血清生化指标、免疫功能和抗氧化能力的影响.

The study was conducted to investigate the effects of dietary marigold lutein on egg quality, serum biochemistry, immune function and antioxidant capacity of laying hens. A total of 432 Roman grey laying hens aged 20-21 weeks were randomly divided into 6 groups. The control group was fed the basal diet, and the positive control group was fed the diet supplemented with 800 mg/kg Marigold lutein (Lidar Company, LDE), and the other 4 groups were experimental groups, there diets were supplemented with 200, 400, 800 mg/kg and 1 600 mg/kg marigold lutein, respectively (Chenguang Company, CG). After 7 days of pre-feeding,the whole experiment last 56 days .The eggs quality was determined on day 28 and day 56, blood samples were collected on day 56 to determine immune indexes, antioxidant capacity and serum biochemical indexes. The results showed as follows: ① In terms of egg quality, the yolk color of all test groups and positive control group except CG-200 group was significantly improved on day 28, and the yolk color of all test groups and positive control group was significantly improved on day 56, but marigold lutein had no significant effect on other eggs quality indexes. ② In terms of serum biochemistry, the content of HDL-C in the CG-1600 group was significantly increased, and the activity of AST was also significantly increased, while the activity of ALT was significantly decreased in the CG-200 group, and ALP activity was significantly increased. ③ In terms of immune function, IgG in serum of laying hens in the CG-200 group and positive control group was significantly increased; IgA in serum of laying hens in the CG-400, CG-1600 groups and positive control group was significantly increased. IgM in serum of laying hens in the CG-800 and CG-1600 groups and positive control group was significantly increased. IL-10 in serum of laying hens in the CG-200 group was significantly increased, while IFN -γ in serum of laying hens in the positive control group was significantly increased. ④ In terms of antioxidant capacity, MDA in serum which in the CG-400 group was significantly decreased; CAT activity in the CG-400 and CG-800 groups was significantly increased; the activity of SOD in serum in the CG-800, CG-1600 groups and positive control group was significantly increased. All the dose groups had no significant effect on the enzyme activity of GSH-Px in serum. In conclusion, Adding proper dosage of marigold lutein in diet can significantly improve the yolk color, and enhance the immune function and the antioxidant capacity of laying hens. [ABSTRACT FROM AUTHOR]