Combined transcriptomic and metabolomic analyses of temperature response of microalgae using waste activated sludge extracts for promising biodiesel production.

• Waste activated sludge extracts can be effectively used to produce algal lipids. • Temperature significantly affected the growth and lipid synthesis of microalgae. • The highest lipid content of 59.13 % was obtained at temperature of 10 °C. • Multi-omics revealed the mechanism of temperature in algal lipid synthesis. • Several genes for lipogenesis, antioxidants, and autophagy were upregulated. Waste activated sludge (WAS) as one of the major pollutants with a significant annual production, has garnered significant attention regarding its treatment and utilization. If improperly discharged, it not only caused environmental pollution but also led to the wastage of valuable resources. In this study, the microalgae growth and lipid accumulation using waste activated sludge extracts (WASE) under different temperature conditions were investigated. The highest lipid content (59.13%) and lipid productivity (80.41 mg L−1 d−1) were obtained at cultivation temperatures of 10 and 25 °C, respectively. It was found that microalgae can effectively utilize TN/TP/NH 4 +-N and other nutrients of WASE. The highest utilization rates of TP, TN and NH 4 +-N were achieved at a cultivation temperature of 10 °C, reaching 84.97, 77.49 and 92.32%, respectively. The algal fatty acids had carbon chains predominantly ranging from C14 to C18, making them suitable for biodiesel production. Additionally, a comprehensive analysis of transcriptomics and metabolomics revealed up-regulation of genes associated with triglyceride assembly, the antioxidant system of algal cells, and cellular autophagy, as well as the accumulation of metabolites related to the tricarboxylic acid (TCA) cycle and lipids. This study offers novel insights into the microscopic mechanisms of microalgae culture using WASE and approaches for the resource utilization of sludge. [Display omitted] [ABSTRACT FROM AUTHOR]