A review on ethanol tolerance mechanisms in yeast: Current knowledge in biotechnological applications and future directions.

Saccharomyces cerevisiae is one of the prominent strains in the brewing and bioethanol industries and has been used for many industrial purposes for ages. Though the organism is an outstanding ethanol producer, the major limiting factor is the stress the organism undergoes during fermentation. One of the significant stresses is the ethanol stress, created by ethanol accumulation in the medium. The ethanol starts to interact with the yeast cell membrane; further, as ethanol concentration increases, it affects a lot of cell organelles. Thereby, cellular activities get disrupted, causing cell death and hence reducing ethanol production. The organism has developed many strategies to overcome this stress by activating the stress response pathway, which regulates many genes involved in modifying the cell membrane cell wall, renaturation of proteins, and altering the metabolism. However, with higher ethanol concentrations, the yeast cells will be unable to tolerate, leading to cell death. Hence, to minimize cell death at higher ethanol concentrations, there is a need to understand the effect of ethanol and its response by the organism; this helps improve the ethanol tolerance of the organism and, thereby, ethanol production. Although many research works are carried out to understand the vital aspect of the tolerance and are reported, very few review papers cover all these points. Hence, this review is designed to include information on all the elements of ethanol tolerance, i.e., ethanol tolerance of different strains of S. cerevisiae , the effect of ethanol on the yeast cells, the mechanism used to tolerate the ethanol, and various techniques developed to improve the ethanol tolerance of the yeast cells. [Display omitted] • Saccharomyces cerevisiae: key in brewing, bioethanol; historic importance; widespread applications. • Detailed exploration of ethanol's harmful effects on yeast, disrupting cellular activities and reducing ethanol production. • Explore ethanol stress adaptations, emphasizing stress response pathway's role in cell component modification. • Addressing the challenge of ethanol tolerance, emphasizing the importance of minimizing cell death for enhanced production. [ABSTRACT FROM AUTHOR]