Universal photoelectrochemical aptasensing platform for antibiotic based on CuInS2/rGO and triplex DNA.

• Triplex DNA molecular switch recognize different targets and release the same probe molecular. • CuInS 2 /rGO was used to enhance the photocurrent signal. • A universal photoelectrochemical aptasensor was constructed for antibiotic highly selective and sensitive detection. In this work, a universal and convenient photoelectrochemical (PEC) biosensor was proposed to detect antibiotic on the basis of CuInS 2 /rGO and triplex DNA molecular switch. High performance photoactive structure was formed via p-type CuInS 2 nanosphere complexed with rGO, which was employed to enhance the cathode photocurrent. Capture probe (CP) was conjugated onto the photoactive interface through covalent bonding. In the absence of target, the triplex DNA (THMS) was in a closed state. After introducing a target, the interaction between the target and the specific ring part DNA fragment leaded to the dissociation of the THMS and thereby liberated the single strand DNA (SP). The above identification process was completed in the homogeneous solution. Then, SP was captured to the electrode by CP and hybridized to form dsDNA. The steric effect of dsDNA hindered the interfacial charge transfer, resulting in signal-off photocurrent output. By only altering the specific loop section sequence of THMS, different targets can release the same SP, and can be detected on the same sensing platform under the same testing conditions. As proof of principle, three kinds of antibiotics amoxicillin (AMOX), ampicillin (AMP) and tobramycin (TOB) was detected, achieving limit of detection values of 3.17 pM, 0.63 nM and 2.81 fM, respectively. This approach was also used to detect antibiotic residues in food and environmental samples, which provides a promising paradigm for developing universal, high-performance and convenient PEC biosensing platform. [ABSTRACT FROM AUTHOR]