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A Modified Cell Culture Method for Autologous Chondrocyte Transplantation.
- Source :
-
Connective Tissue Research . Mar2005, Vol. 46 Issue 2, p93-99. 7p. 1 Diagram, 1 Chart, 1 Graph. - Publication Year :
- 2005
-
Abstract
- Autologous chondrocyte transplantation (ACT) is a promising method to treat chondral and osteochondral defects. This study introduced a modified method for cell culture in ACT. Porcine chondrocytes were cultured for 3 weeks under low hydrostatic pressure at 250 Pa. The results showed that the dry weight of the cartilage-like membrane in the loading group was 3.0 times more than the control group (no loading) ( p ), and cell numbers were significantly increased by 3.1 times ( p ) after a 3-week culture. Compared with the fresh tissue sample, the mRNA expression of collagen II was not statistically different and the mRNA of aggrecan was only slightly decreased by 19%. These data suggest that the hydrostatic pressure at this level significantly increased the cell numbers and biosynthesis of cultured chondrocytes. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03008207
- Volume :
- 46
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Connective Tissue Research
- Publication Type :
- Academic Journal
- Accession number :
- 17552414
- Full Text :
- https://doi.org/10.1080/03008200590954122