Vector-borne pathogen surveillance in a metagenomic world.

This article discusses the challenges and opportunities in detecting vector-borne pathogens using nucleic acid-based assays and metagenomic sequencing. The authors highlight the difficulties in distinguishing pathogens from closely related endosymbionts and the need for regular updating of detection approaches. They also emphasize the importance of reviewing and improving primer and probe sequences, as well as considering the integration of endosymbiont sequences into host genomes. The article further discusses the challenges in detecting and identifying viruses, as well as the limitations of NGS technologies and computational analysis in microbial detection. The authors caution that the biological relevance of microbial detection should be carefully considered, especially in vector and environmental samples, and that additional evidence may be required to establish causative associations with disease. They conclude by emphasizing the need for careful planning and consideration of surveillance goals within the One Health concept. [Extracted from the article]