Back to Search
Start Over
Enzymatic characterization and application of soybean hull peroxidase as an efficient and renewable biocatalyst for degradation of zearalenone.
- Source :
-
International Journal of Biological Macromolecules . Mar2024:Part 2, Vol. 260, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
-
Abstract
- Zearalenone (ZEN) is a notorious mycotoxin commonly found in Fusarium -contaminated crops, which causes great loss in livestock farming and serious health problems to humans. In the present work, we found that crude peroxidase extraction from soybean hulls could use H 2 O 2 as a co-substate to oxidize ZEN. Molecular docking and dynamic simulation also supported that ZEN could bind to the active site of soybean hull peroxidase (SHP). Subsequently, SHP extracted from soybean hulls was purified using a combined purification protocol involving ammonium sulfate precipitation, ion exchange chromatography and size exclusion chromatography. The purified SHP showed wide pH resistance and high thermal stability. This peroxidase could degrade 95 % of ZEN in buffer with stepwise addition of 100 μM H 2 O 2 in 1 h. The two main ZEN degradation products were identified as 13-OH-ZEN and 13-OH-ZEN-quinone. Moreover, SHP-catalyzed ZEN degradation products displayed much less cytotoxicity to human liver cells than ZEN. The application of SHP in various food matrices obtained 54 % to 85 % ZEN degradation. The findings in this study will promote the utilization of SHP as a cheap and renewable biocatalyst for degrading ZEN in food. • SHP could directly oxidize ZEN with H 2 O 2 as a co-substrate. • 13-OH-ZEN and 13-OH-ZEN-quinone were the main ZEN oxidation products. • SHP-catalyzed ZEN degradation could be regarded as detoxification. • The application of SHP in food matrices obtained 54 % to 85 % ZEN degradation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01418130
- Volume :
- 260
- Database :
- Academic Search Index
- Journal :
- International Journal of Biological Macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 175637974
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2024.129664