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Pathological α-synuclein detected by real-time quaking-induced conversion in synucleinopathies.
- Source :
-
Experimental Gerontology . Mar2024, Vol. 187, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
-
Abstract
- synucleinopathies are diseases characterized by the aggregation of α-synuclein (α-syn), which forms fibrils through misfolding and accumulates in a prion-like manner. To detect the presence of these α-syn aggregates in clinical samples, seed amplification assays (SAAs) have been developed. These SAAs are capable of amplifying the α-syn seeds, allowing for their detection. αSyn-SAAs have been reported under the names 'protein misfolding cyclic amplification' (αSyn-PMCA) and 'real-time quaking-induced conversion'α-Syn-RT-QuIC. The α-Syn RT-QuIC, in particular, has been adapted to amplify and detect α-syn aggregates in various biospecimens, including cerebrospinal fluid (CSF), skin, nasal brushing, serum and saliva. The α-syn RT-QuIC assay has demonstrated good sensitivity and specificity in detecting pathological α-syn, particularly in Parkinson's disease (PD) and dementia with Lewy bodies (DLB) cases, with an accuracy rate of up to 80 %. Additionally, differential diagnosis between DLB and PD, as well as PD and multiple system atrophy (MSA), can be achieved by utilizing certain kinetic thioflavin T (ThT) parameters and other parameters. Moreover, the positive detection of α-syn in the prodromal stage of synucleinopathies provides an opportunity for early intervention and management. In summary, the development of the α-syn RT-QuIC assay has greatly contributed to the field of synucleinopathies. Therefore, we review the development of α-syn RT-QuIC assay and describe in detail the recent advancements of α-syn RT-QuIC assay for detecting pathological α-syn in synucleinopathies. • Real-time quaking-induced conversion (RT-QuIC) has been adapted to amplify and detect α-syn aggregates in various biospecimens. • α-syn RT-QuIC has shown high sensitivity and specificity in diagnosising α-synucleinopathies. • α-syn RT-QuIC helps identifying individuals in the prodromal stage of α-synucleinopathies. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 05315565
- Volume :
- 187
- Database :
- Academic Search Index
- Journal :
- Experimental Gerontology
- Publication Type :
- Academic Journal
- Accession number :
- 175642073
- Full Text :
- https://doi.org/10.1016/j.exger.2024.112366