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Topographic Distribution of miRNAs (miR-30a, miR-223, miR-let-7a, miR-let-7f, miR-451, and miR-486) in the Plasma Extracellular Vesicles.

Authors :
Petrova, Tatiana
Kalinina, Olga
Aquino, Arthur
Grigoryev, Evgeniy
Dubashynskaya, Natallia V.
Zubkova, Kseniya
Kostareva, Anna
Golovkin, Alexey
Source :
Non-Coding RNA. Feb2024, Vol. 10 Issue 1, p15. 25p.
Publication Year :
2024

Abstract

There are many articles on the quantitative analysis of miRNAs contained in a population of EVs of different sizes under various physiological and pathological conditions. For such analysis, it is important to correctly quantify the miRNA contents of EVs. It should be considered that quantification is skewed depending on the isolation protocol, and different miRNAs are degraded by nucleases with different efficiencies. In addition, it is important to consider the contribution of miRNAs coprecipitating with the EVs population, because the amount of miRNAs in the EVs population under study is skewed without appropriate enzymatic treatment. By studying a population of EVs from the blood plasma of healthy donors, we found that the absolute amount of miRNA inside the vesicles is commensurate with the amount of the same type of miRNA adhered to the outside of the EVs. The inside/outside ratio ranged from 1.02 to 2.64 for different investigated miRNAs. According to our results, we propose the hypothesis that high occupancy of miRNAs on the outer surface of EVs influence on the transporting RNA repertoire no less than the inner cargo received from the host cell. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
2311553X
Volume :
10
Issue :
1
Database :
Academic Search Index
Journal :
Non-Coding RNA
Publication Type :
Academic Journal
Accession number :
175646907
Full Text :
https://doi.org/10.3390/ncrna10010015