Potential of Surrogate Matrix for the Quantification of S-Adenosylmethionine and S-Adenosylhomocysteine in Human Plasma by Means of Tandem Liquid Chromatography–Mass Spectrometry with Selected Reaction Monitoring.

Despite the existing knowledge regarding the metabolic dysregulation of sulfur-containing amino acids in athletes during intense physical exercise, the precise role of S-adenosylmethionine and S-adenosylhomocysteine as biomarkers for adaptive changes remains unresolved. The challenge in addressing this issue lies in the substantial impact of matrix effects on quantitative outcomes under electrospray ionization conditions. To overcome this issue, we propose the adoption of a surrogate matrix for constructing calibration curves. In our study, a surrogate matrix was prepared by incubating pooled plasma from volunteers for 120 h at 37°C. By utilizing external calibration with a surrogate matrix and the standard addition method, we achieved a maximum concentration variation of 4 ng mL–1 for the determination of both S-adenosylmethionine and S-adenosylhomocysteine. [ABSTRACT FROM AUTHOR]