Insight into microbial adaptability in continuous flow anaerobic ammonium oxidation process for low-strength sewage treatment.

[Display omitted] • Efficient sewage treatment was developed in continuous flow anammox process. • EPS content plays a key role in the formation of microbial interactions. • The inhibitory effect of DNB to NOB can be induced by organic substances. • The DNB that uses slowly biodegradable organics promotes anammox under limited NH 4 +-N. Organic matter concentration is a critical factor influencing the adaptability of anaerobic ammonium oxidation (anammox) bacteria to low-strength sewage treatment. To address this challenge and achieve stable anammox activity, a micro-aeration partial nitrification-anammox process was developed for continuous-flow municipal sewage treatment. Under limited ammonium conditions, the effective utilization of organics in denitrification promoted the stable accumulation of nitrite and enhanced anammox activity. This, in turn, led to enhanced nitrogen removal efficiency, reaching approximately 87.7%. During the start-up phase, the protein content of extracellular polymeric substances (EPS) increased. This enhanced EPS intensified the inhibitory effect of denitrifying bacteria (DNB) on nitrite-oxidizing bacteria through competition for nitrite, thereby facilitating the proliferation of anammox bacteria (AnAOB). Additionally, several types of DNB capable of utilizing slowly biodegradable organics contributed to the adaptability of AnAOB. These findings provide valuable insights for ensuring efficient anammox performance and robust nitrogen removal in the treatment of low-strength sewage. [ABSTRACT FROM AUTHOR]