Immunohistochemical Detection of Tissue Localization of Acute Phase Proteins in Cattle Pneumonic Pasteurellosis.

This study was aimed to determine by immunohistochemical methods the expressions of acute phase protein such as Haptoglobin, C-reactive protein and Serum Amyloid in the lungs of cattle suffering from pneumonic pasteurellosis (PP). The material of the study was the lungs of 8-18 month old cattle Holstein with Fibrinous Bronchopneumonia brought for necropsy to the Pathology Department of the Selcuk University Veterinary Faculty between 2019-2020. As a result of microbiological culture from the lungs of these animals, 6 lungs where Pasteurella spp. was isolated were used in the study. Additionally, 6 culture-negative healthy animals obtained from the nearby slaughterhouse were used for lung control. A total of 12 bovine lung samples were subjected to histopathological and immunohistochemical staining. In the histopathological examination, while no microscopic lesions were observed in the lungs of the control group, inflammatory hyperemia and red and grey consolidation stages of fibrinous pneumonia were detected in animals with pneumonic pasteurellosis. Immunohistochemically, immunoreactivity was very mild or without staining for primers in healthy lungs. Animals with pneumonic pasteurellosis showed immunopositivity in haptoglobin (Hp), oedema fluid and inflammatory cell infiltrations (P>0.05). C-reactive protein (CRP) staining showed expression in all criteria (epithelial, capillary, oedema and inflammatory cell infiltration) at all phases, and the highest mean score was found during the red consolidation phase (P<0.01). Serum Amyloid A (SAA) staining showed immunopositive staining in oedema fluid and inflammatory cell infiltrations, and the highest mean scores were during the red and grey consolidation phases (P<0.01). According to the results of the study, it can be considered that acute phase proteins may be important in the development process and/or pathophysiology of the disease, and SAA and CRP may provide more sensitive results for the diagnosis and prognosis of the disease. [ABSTRACT FROM AUTHOR]