Back to Search
Start Over
Hyperglycemia modulates M1/M2 macrophage polarization in chronic diabetic patients with pulmonary tuberculosis infection.
- Source :
-
Immunobiology . Mar2024, Vol. 229 Issue 2, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
-
Abstract
- • PTB + DM displayed skewed cytokine levels with reduced IFN-γ and iNOSbut higher IL-1β and CD206, which may lead to increased TB pathology. • IL-1β and CD206 correlate with HbA1c in PTB + DM and DM groups, suggesting linkbetween glycemic control and cytokine dynamics. • M1 markers, namely IL-1β and iNOS, are significantly associated with sputum positivity in both PTB and PTB + DM cases. Increased susceptibility to bacterial infections like tuberculosis (TB) is one of the complications of type 2 diabetes, however the underlying mechanisms remains poorly characterized. To explore how chronic hyperglycemia in diabetes affects progression of active TB, we examined mRNA expression of M1 (proinflammatory) and M2 (anti-inflammatory) cytokines/markers, in monocyte-derived macrophages obtained from patients with PTB + DM (pulmonary TB + diabetes mellitus type 2), patients with DM alone, patients with PTB alone, and healthy individuals (controls). Our findings indicate a dysregulated cytokine response in patients with both PTB and DM, characterized by decreased expression levels of interferon-gamma (IFN-γ) and inducible nitric oxide synthase (iNOS), along with increased expression levels of interleukin-1 beta (IL-1β) and CD206. Furthermore, we observed a positive correlation of IL-1β and CD206 expression with levels of glycosylated hemoglobin (HbA1c) in both PTB + DM and DM groups, while IFN-γ showed a positive correlation with HbA1c levels, specifically in the PTB + DM group. Additionally, M1 cytokines/markers, IL-1β and iNOS were found to be significantly associated with the extent of sputum positivity in both PTB and PTB + DM groups, suggesting it to be a function of increased bacterial load and hence severity of infection. Our data demonstrates that tuberculosis in individuals with PTB + DM is characterized by altered M1/M2 cytokine responses, indicating that chronic inflammation associated with type 2 diabetes may contribute to increased immune pathology and inadequate control of tuberculosis infection. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01712985
- Volume :
- 229
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Immunobiology
- Publication Type :
- Academic Journal
- Accession number :
- 175725159
- Full Text :
- https://doi.org/10.1016/j.imbio.2024.152787