Low alkalinity, free ammonia, and free nitrous acid cooperatively stabilize partial nitrification under excessive aeration condition.

In this study, a lab-scale sequencing batch reactor (SBR) was established to assess the feasibility of maintaining partial nitrification in treating high-ammonium wastewater under the condition of excessive aeration (1 L/min, 1289 min). Results showed that the average ammonia (NH 4 +-N) removal efficiency (ARE) was 93.4 ± 2.7% and the average nitrite accumulation ratio (NAR) was 90.7 ± 2.8% during 15–50 d. In a typical cycle, free ammonia (FA) and free nitrous acid (FNA) alternately inhibited the activity of nitrite oxidizing bacteria (NOB), while low alkalinity limited further nitrification at even excessive aeration. Metagenomic analysis revealed that the relative abundance of Nitrosomonas , as the ammonia oxidizing bacteria (AOB), was up to 1.61%, while NOB were not detected. Taken together, this study indicated partial nitrification was almost not affected by excessive aeration, demonstrating its robustness depending on the cooperative strategy of the low alkalinity limitation and the inhibition of FA and FNA. [Display omitted] • Combined inhibition of FA and FNA resulted in the wash-out of NOB. • Low alkalinity limited further oxidation of NO 2 −-N caused by excessive aeration. • Nitrite accumulation ratio of 91.7% was maintained under excessive aeration condition. • Denitrification providing alkalinity for PN ensured high ARE and NAR concurrently. • Nitrosomonas (1.6%) was identified as the exclusive genus of AOB in the PN-SBR. [ABSTRACT FROM AUTHOR]