LAMP3 inhibited the proliferation, metastatic and PC-3-induced vasculogenesis of HUVEC by regulating VEGF/AKT signaling.

Objective To explore the impact of Lysosome-Associated Membrane Protein 3 (LAMP3) on th eproliferation, migration and angiogenesis of PC-3 cells. Methods LAMP3 expression in normal prostate epithelial cells and prostate cancer bone metastasis cells was detected using western blot and RT-PCR. Stable LAMP3-silenced PC-3 cells were constructed, and the effects of LAMP3 on proliferation, invasion, and migration of PC-3 cells were assessed using CCK8, scratch assay, and transwell assay, respectively. ELISA and angiogenesis assays were employed to examine the expression of VEGF and MMP9, as well as angiogenesis of HUVEC cells induced by PC-3 cells. Finally, WB and RT-PCR were used to detect the expression of VEGF, AKT/p-AKT. Results Our findings showed that the expression level of LAMP3 was significantly higherin prostate cellsthan in normal prostate epithelial cells, especially in PC-3 cells (P < 0.05). We also found that silencing LAMP3 could inhibit the proliferation, migration and invasion of PC-3 cells, along with the expression of VEGF and MMP9 and the PC-3 cells-induced angiogenesis, and these results were statistically significant (P < 0.05). Furthermore, LAMP3 downregulated the expression of VEGF and AKT/p-AKT in PC-3 cells. Conclusion LAMP3 can affect the proliferation, migrationand angiogenesis of PC-3 cells through the regulation of VEGF/AKT pathway. Thus, LAMP3 might be a potential therapeutic target for prostate cancer bone metastasis. [ABSTRACT FROM AUTHOR]