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The stiffness and collagen control differentiation of osteoclasts with an altered expression of c-Src in podosome.
- Source :
-
Biochemical & Biophysical Research Communications . Apr2024, Vol. 704, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
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Abstract
- Osteoclasts are hematopoietic cells attached to the bones containing type I collagen-deposited hydroxyapatite during bone resorption. Two major elements determine the stiffness of bones: regular calcified bone (bone that is resorbable by osteoclasts) and un-calcified osteoid bone (bone that is un-resorbable by osteoclasts). The osteolytic cytokine RANKL promotes osteoclast differentiation; however, the roles of the physical interactions of osteoclasts with calcified and un-calcified bone at the sealing zones and the subsequent cellular signaling remain unclear. In this study, we investigated podosomes, actin-rich adhesion structures (actin-ring) in the sealing zone that participates in sensing hard stiffness with collagen in the physical environment during osteoclast differentiation. RANKL-induced osteoclast differentiation induction was promoted when Raw264.7 cells were cultured on collagen-coated plastic dishes but not on non-coated plastic dishes, which was associated with the increased expression of podosome-related genes and Src. In contrast, when cells were cultured on collagen gel, expression of podosome-related genes and Src were not upregulated. The induction of podosome-related genes and Src requires hard stiffness with RGD-containing substratum and integrin-mediated F-actin polymerization. These results indicate that osteoclasts sense both the RGD sequence and stiffness of calcified collagen through their podosome components regulating osteoclast differentiation via the c-Src pathway. • Podosomes are actin-rich adhesion structures in the sealing zone of osteoclast. • Osteoclasts sense both the RGD and stiffness of calcified collagen by podosomes. • Osteoclast differentiation is associated with Src pathway in podosome. [ABSTRACT FROM AUTHOR]
- Subjects :
- *OSTEOCLASTS
*SRC gene
*COLLAGEN
*BONE resorption
*CELL communication
*BONE cells
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 704
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 175938970
- Full Text :
- https://doi.org/10.1016/j.bbrc.2024.149636