Back to Search
Start Over
High-affinity truncated aptamers for detection of Cronobacter spp with magnetic separation-assisted DNAzyme-driven 3D DNA walker.
- Source :
-
Microchimica Acta . Mar2024, Vol. 191 Issue 3, p1-13. 13p. - Publication Year :
- 2024
-
Abstract
- After optimizing the original aptamer sequence by truncation strategy, a magnetic separation-assisted DNAzyme-driven 3D DNA walker fluorescent aptasensor was developed for detecting the food-borne pathogen Cronobacter species. Iron oxide magnetic nanoparticles (MNPs) modified with a hybrid of truncated aptamer probe and DNAzyme strand (AP-E1) denoted as MNPs@AP-E1, were employed as capture probes. Simultaneously, a DNAzyme-driven 3D-DNA walker was utilized as the signal amplification element. The substrate strand (Sub) was conjugated with the gold nanoparticles (AuNPs), resulting in the formation of AuNPs@Sub, which served as a 3D walking track. In the presence of the target bacteria and Mg2+, E1-DNAzyme was activated and moved along AuNPs@Sub, continuously releasing the signal probe. Under optimized conditions, a strong linear correlation was observed for Cronobacter sakazakii (C. sakazakii) in the concentration range 101 to 106 CFU mL−1, with a low detection limit of 2 CFU mL−1. The fluorescence signal responses for different Cronobacter species exhibited insignificant differences, with a relative standard deviation of 3.6%. Moreover, the aptasensor was successfully applied to determine C. sakazakii in real samples with recoveries of 92.86%—108.33%. Therefore, the novel method could be a good candidate for ultra-sensitive and selective detection of Cronobacter species without complex manipulation. [ABSTRACT FROM AUTHOR]
- Subjects :
- *APTAMERS
*CRONOBACTER
*IRON oxide nanoparticles
*GOLD nanoparticles
*TRAILS
*DNA
Subjects
Details
- Language :
- English
- ISSN :
- 00263672
- Volume :
- 191
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Microchimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 176101445
- Full Text :
- https://doi.org/10.1007/s00604-024-06199-2