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Development and validation of LC-MS/MS for quantifying omadacycline from stool for gut microbiome studies.
- Source :
-
Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences . Apr2024, Vol. 1236, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
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Abstract
- • Antimicrobial pharmacokinetics is an emerging area of gut microbiome research. • Omadacyline is a tetracycline-class antibiotic with potent activity against Clostridioides difficile and a low propensity to cause C. difficile infection. • This study developed and validated a LC-MS/MS method to quantify omadacycline and its epimerization in stool. • A remarkable epimerization of omadacycline was observed in stool samples. This study developed and validated a new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantify omadacycline and its epimerization in stool to facilitate microbiome studies. Omadacycline was extracted in a methanol-water-ethylenediaminetetraacetic acid (ETDA) solvent containing deuterated omadacycline as internal standard, followed by dilution. In an optimal gradient elution mode, omadacycline and its C4 epimer were separated within 5 min on reversed-phase C18 column. The method showed a broad working range of 0.1–200 ng/ml with a limitation of detection (LOD) of 0.03 ng/ml, little fecal matrix effect, good intra-day and inter-day accuracy (90–101 %), precision (2–15 %), and recovery rate (99–105 %). The method was sufficiently sensitive to quantify omadacycline in human fecal samples (n = 82) collected during a 10-day therapy course and at follow-up (day 13 and day 30) that ranged from 1 to 4785 µg/g. Further analysis revealed that ∼9 % of omadacycline was epimerized in fecal matrix control while, on average, 37.4 % was epimerized in human fecal samples. This study developed and validated a novel, simple, sensitive, and accurate method utilizing LC-MS/MS to quantify omadacycline its epimerization in the human gut. This has important implications for future studies of omadacycline and other tetracycline-class antibiotics as part of gut microbiome studies. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15700232
- Volume :
- 1236
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 176121975
- Full Text :
- https://doi.org/10.1016/j.jchromb.2024.124057