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Integrated microfabricated systems including a purification module and an on-chip nano electrospray ionization interface for biological analysis
- Source :
-
Journal of Chromatography A . Apr2005, Vol. 1071 Issue 1/2, p213-222. 10p. - Publication Year :
- 2005
-
Abstract
- Abstract: We report here on an integrated microfabricated device dedicated to the preparation of biological samples prior to their on-line analysis by electrospray ionization-mass spectrometry (ESI-MS). This microfluidic device is fabricated using the negative photoresist SU-8 by microtechnology techniques. The device includes a chromatographic module plus an ESI interface for MS. The chromatographic module is dedicated to sample purification and is based on a polymer monolithic phase which includes hydrophobic moieties. The ESI interface is integrated onto the chip and is based on a capillary slot. We present here the integration of these different modules onto a single system that is fabricated via a SU-8-based microtechnology route. We present also their testing for the purification of peptide samples. This started with a partial integration step with the combination of at least two of the modules (microsystem+monolith; microsystem+nib) and their test before the fabrication and testing of fully integrated microsystems. [Copyright &y& Elsevier]
- Subjects :
- *MASS spectrometry
*SPECTRUM analysis
*PHOTORESISTS
*IONIZATION (Atomic physics)
Subjects
Details
- Language :
- English
- ISSN :
- 00219673
- Volume :
- 1071
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatography A
- Publication Type :
- Academic Journal
- Accession number :
- 17612928
- Full Text :
- https://doi.org/10.1016/j.chroma.2004.12.037