急性髓系白血病细胞条件培养液对间充质干细胞的生物学影响.

BACKGROUND: At present, the biological functions and molecular changes of bone marrow mesenchymal stem cells in the tumor microenvironment of acute myeloid leukemia are still unclear. OBJECTIVE: To explore the changes in the biological function of bone marrow mesenchymal stem cells in acute myeloid leukemia and the role of acute myeloid leukemia conditioned medium by bioinformatics and experiment. METHODS: Differential genes were screened from GEO data sets, and enrichment analysis was performed. The protein-protein interaction network was constructed and the Hub gene was obtained. Bone marrow mesenchymal stem cells from patients with acute myeloid leukemia and healthy donors were cultured. Bone marrow mesenchymal stem cells from healthy donors were treated with acute myeloid leukemia conditioned culture solution. Each group was subjected to the adipogenic differentiation, osteogenic differentiation, staining of β-galactosidase, detection of the cell cycle, and validation of Hub genes. RESULTS AND CONCLUSION: (1) Gene expression data of bone marrow mesenchymal stem cells from acute myeloid leukemia patients and healthy donors were obtained from GSE84881, and 184 up-regulated genes and 140 down-regulated genes were screened. (2) The biological functions of enrichment mainly include cell cycle, adipocyte differentiation, cell metabolism, and MYC pathway. According to the Degree algorithm, 10 up-regulated Hub genes and 10 downregulated Hub genes were selected. (3) The cell in vitro experiment found that: compared with the control group, the surface antigen of acute myeloid leukemia mesenchymal stem cells did not change, but it showed enhanced lipid differentiation ability, weakened osteogenic differentiation ability, increased β-galactosidase positive cell number, altered cell morphology, arrested cell cycle, increased LGALS3 expression, and decreased MYC expression. Mesenchymal stem cells from healthy donors showed similar changes after being cultured in acute myeloid leukemia conditioned medium. (4) The results show that biological function of mesenchymal stem cells is altered in the acute myeloid leukemia microenvironment, which provides new insights into the interaction between mesenchymal stem cells and tumor cells. [ABSTRACT FROM AUTHOR]