Study on quantitative detection of bacterial endotoxin content in cat food using dynamic turbidity method.

The experiment aimed to establish a method for quantitatively measuring the concentration of bacterial endotoxins in cat food using the dynamic turbidity method. Bacterial endotoxins in cat food samples were extracted with water, centrifuged, filtered, and the filtrate was appropriately diluted. Quantitative determination was carried out using the dynamic turbidity method on a bacterial endotoxin measuring instrument. The results showed good linearity of bacterial endotoxin within the range of 0.040 to 5.000 EU/mL (correlation coefficient r=-0.995 3, >0.980). The standard curve was effective, and the relative standard deviation (RSD) of repeated measurements ranged from 8.9% to 14.0% ( n=6). The recovery rates of interference tests were between 50% and 200% when the dilution factor was ≥1 000. The limit of quantification (LOQ) of the method was determined as 0.04 EU/mg. The study indicates that the method is simple, reliable, accurate, and exhibits good reproducibility, making it effective for detecting bacterial endotoxin content in cat food samples, with promising application prospects. [ABSTRACT FROM AUTHOR]