A mitochondria-targeting fluorescent probe for the dual-emission fluorescence-enhanced detection of hydrogen sulfide and turn-on detection of hydrazine.

A fluorescent probe (probe 1) with selectivity and sensitivity for H 2 S and N 2 H 4 has been developed. Probe 1 exhibits dual-excitation and dual-emission fluorescence response to H 2 S and fluorescence enhancement toward N 2 H 4 with distinct naked-eye color changes. Moreover, probe 1 displayed low detection limits for H 2 S and N 2 H 4 of 0.90 nM and 23.6 nM, respectively. The reaction mechanism of probe 1 toward H 2 S and N 2 H 4 was established using 1H NMR and HR-MS spectroscopy. Additionally, probe 1 was used for monitoring and differentiating H 2 S and N 2 H 4 in HeLa cells and displays good biocompatibility and low cytotoxicity. • Probe 1 exhibits dual-emission fluorescence-enhanced detection of H 2 S in mitochondria. • Probe 1 exhibits a turn-on response for the detection of N 2 H 4 in mitochondria. • Probe 1 exhibits rapid response, good optical performance, high selectivity, and high sensitivity in solution. • Probe 1 displays multi species detection in the mitochondria of HeLa cells and zebrafish. • Detection using Probe 1 can be achieved using a smartphone with a chromaticity APP. [ABSTRACT FROM AUTHOR]