A rapid one-step affinity purification of C-phycocyanin from Spirulina platensis.

• Developed an affinity chromatography for high purity C-phycocyanin purification. • Using computer-simulated molecular docking for screening ligand. • Achieved reagent-grade C-phycocyanin in a single purification step. • The highest purity of C-phycocyanin was recorded as 4.53. • The highest yield of C-phycocyanin was recorded at 69 %. The high-purity phycocyanin has a high commercial value. Most current purification methods of C-phycocyanin involve multiple steps, which are complicated and time-consuming. To solve the problem, this research was studied, and an efficient affinity chromatography purification for C-phycocyanin from Spirulina platensis was developed. Through molecular docking simulation, virtual screening of ligands was performed, and ursolic acid was identified as the specific affinity ligand, which coupled to Affi-Gel 102 gel via 1-ethyl (3-dimethylaminopropyl)-3-carbodiimide, hydrochloride as coupling agent. With this customized and synthesized resin, a high-efficiency one-step purification procedure for C-phycocyanin was developed and optimized, the purity was determined to be 4.53, and the yield was 69 %. This one-step purification protocol provides a new approach for purifying other phycobilin proteins. [ABSTRACT FROM AUTHOR]