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Exploring the potential of Mustard (Brassica spp.) seeds through 'Kolhu' traditional method of extraction and novel identification of an anti-cancer dipeptide, Aurantiamide acetate (Asperglaucide) on ultra-performance liquid chromatography-mass spectrometry coupled with quadrupole time-of-flight (UPLC/MS-QToF) analytical platform

Authors :
Balkrishna, Acharya
Verma, Sudeep
Priya Rani, M.
Nain, Pardeep
Varshney, Anurag
Source :
Food Chemistry. Jul2024, Vol. 446, pN.PAG-N.PAG. 1p.
Publication Year :
2024

Abstract

[Display omitted] • Exploration of anticancer dipeptide Aurantiamide acetate from the seeds of Brassica species. • Traditional 'Kolhu' method showed remarkable efficiency in extracting dipeptides. • Phytometabolite profiling was done by a validated UPLC-MS/QToF analytical method. • This novel method was found to be robust for the effective quantitation of dipeptides. Mustard (Brassica spp.) is one of the world's oldest condiments in the food basket, which holds a significant place in the global culinary landscape due to historical prominence and perceived health benefits. This study explores the extraction of oils from Mustard seeds by employing traditional 'Kolhu' method, modern supercritical fluid, and solvent extraction techniques. This study, for the first-time, identified Aurantiamide acetate, a potent anti-cancer dipeptide in Mustard seeds using ultra-performance liquid chromatography-mass spectrometry coupled with quadrupole time-of-flight (UPLC/MS-QToF) analytical platform. The analytical methodology was meticulously validated encompassing optimal parameters such as limit of detection, limit of quantification, precision, accuracy, linearity and robustness, within the range. Interestingly, 'Kolhu' method of oil extraction exhibited better yield of Aurantiamide acetate, suggesting superior efficiency of traditional methods. This study accentuates the importance of classical extraction methods, used traditionally, and emphasizes that naturally occurring substances indeed could be harnessed for better health. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
03088146
Volume :
446
Database :
Academic Search Index
Journal :
Food Chemistry
Publication Type :
Academic Journal
Accession number :
176268856
Full Text :
https://doi.org/10.1016/j.foodchem.2024.138870