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Rapid and sensitive quantitation of amitraz in orange, tomato, and eggplant samples using immunochromatographic assay.
- Source :
-
Food Chemistry . Jul2024, Vol. 446, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
-
Abstract
- • A novel AMT hapten was designed by adding a carboxyl group with 4-C spacer arms. • The sensitive and specific anti-AMT mAb was prepared based on hapten design. • The immunochromatographic assay was firstly developed for AMT rapid detection. • The established mehod can meet the requirements of MRLs for AMT residues in foods. Amitraz (AMT) is a broad-spectrum formamidine insecticide and acaricide. In this study, we produced an anti-AMT monoclonal antibody (mAb) with high performance. The half-maximal inhibitory concentration of the anti-AMT mAb was 4.418 ng/mL, the cross reactivity with other insecticides was negligible, and an affinity constant was 2.06 × 109 mmol/L. Additionally, we developed an immunochromatographic assay for the rapid detection of AMT residues in oranges, tomatoes, and eggplants. The cut-off values were 2000 μg/kg in oranges and tomato samples and 1000 μg/kg in eggplant samples and the calculated limits of detection were 14.521 μg/kg, 6.281 μg/kg, and 3.518 μg/kg in oranges, tomatoes, and eggplants, respectively, meeting the detection requirements for AMT in fruits and vegetables. The recovery rates ranged between 95.8 % and 105.2 %, consistent with the recovery rates obtained via LC-MS/MS. Our developed immunochromatographic assay can effectively, accurately, and rapidly determine AMT residues in oranges, tomatoes, and eggplants. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03088146
- Volume :
- 446
- Database :
- Academic Search Index
- Journal :
- Food Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 176268878
- Full Text :
- https://doi.org/10.1016/j.foodchem.2024.138899