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Prevalence, Molecular Characteristics and Virulence Identification of Bovine Parainfluenza Virus Type 3 in China.

Authors :
Xu, Xiaowen
Zhao, Wanyue
Xiang, Zhijie
Wang, Chen
Qi, Mingpu
Zhang, Sen
Geng, Yuanchen
Zhao, Yuhao
Yang, Kaihui
Zhang, Yanan
Guo, Aizhen
Chen, Yingyu
Source :
Viruses (1999-4915). Mar024, Vol. 16 Issue 3, p402. 19p.
Publication Year :
2024

Abstract

Bovine parainfluenza virus type 3 (BPIV-3) is one of the major pathogens of the bovine respiratory disease complex (BRDC). BPIV-3 surveillance in China has been quite limited. In this study, we used PCR to test 302 cattle in China, and found that the positive rate was 4.64% and the herd-level positive rate was 13.16%. Six BPIV-3C strains were isolated and confirmed by electron microscopy, and their titers were determined. Three were sequenced by next-generation sequencing (NGS). Phylogenetic analyses showed that all isolates were most closely related to strain NX49 from Ningxia; the genetic diversity of genotype C strains was lower than strains of genotypes A and B; the HN, P, and N genes were more suitable for genotyping and evolutionary analyses of BPIV-3. Protein variation analyses showed that all isolates had mutations at amino acid sites in the proteins HN, M, F, and L. Genetic recombination analyses provided evidence for homologous recombination of BPIV-3 of bovine origin. The virulence experiment indicated that strain Hubei-03 had the highest pathogenicity and could be used as a vaccine candidate. These findings apply an important basis for the precise control of BPIV-3 in China. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
19994915
Volume :
16
Issue :
3
Database :
Academic Search Index
Journal :
Viruses (1999-4915)
Publication Type :
Academic Journal
Accession number :
176333822
Full Text :
https://doi.org/10.3390/v16030402