Development of an automation system for express analysis of african and classical swine fever antigens.

The epizootic situation in Russia and the world remains unstable for a number of infectious diseases, including classical swine fever and African swine fever. Classical and European swine fever is an infectious highly contagious disease of pigs, characterized by fever, septicaemia, haemorrhagic diathesis, krupsy colitis and enteritis, as well as krupsy pneumonia. In diseased pigs, the pathogen is present in all organs and tissues. African swine fever and Montgomery's disease are highly contagious and acute septic diseases of pigs, characterised by fever, haemorrhagic inflammation of various organs and high mortality. These diseases cause great economic damage, sick animals are eliminated and veterinary and sanitary and other measures are necessary. Studies on the epizootology of the spread of African swine fever and the monitoring of the epidemiological situation have been carried out in Russia over the past decade. In 2021, about two hundred episodes were recorded in the Russian Federation. The peculiarities of this virus include delayed formation of antibodies and immunity in pigs. The virus affects bone marrow cells and is found in the blood leucocytes of healthy pigs. At present, multiplex polymerase chain reaction tests for diagnosis of classical swine fever, African swine fever and other viruses have been developed for use by veterinary virologists. An automated polymerase chain reaction-based diagnosis system has been developed. The main way to determine RNA of classical swine fever virus and DNA of African swine fever virus is serological examination. The article proposes the development of an automated system using immunochromatographic analysis on the basis of special probes containing monoclonal antibodies for plague viruses. Software-hardware using PLC Omron was developed. A simulation of the software showed satisfactory operation of the system. Timely detection of plague viruses in pigs will help to prevent the development of the epidemic and improve the epidemiological situation in the Russian Federation. [ABSTRACT FROM AUTHOR]